Transformation of the Green Alga Haematococcus pluvialis with a Phytoene Desaturase for Accelerated Astaxanthin Biosynthesis
نویسندگان
چکیده
منابع مشابه
Transformation of the green alga Haematococcus pluvialis with a phytoene desaturase for accelerated astaxanthin biosynthesis.
Astaxanthin is a high-value carotenoid which is used as a pigmentation source in fish aquaculture. Additionally, a beneficial role of astaxanthin as a food supplement for humans has been suggested. The unicellular alga Haematococcus pluvialis is a suitable biological source for astaxanthin production. In the context of the strong biotechnological relevance of H. pluvialis, we developed a geneti...
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The green unicellular alga, Haematococcus pluvialis has two antioxidative mechanisms against environmental oxidative stress: antioxidative enzymes in vegetative cells and the antioxidative ketocarotenoid, astaxanthin, in cyst cells. We added a reagent that generates superoxide anion radicals (O2 ), methyl viologen, to mature and immature cysts of H. pluvialis. Tolerance to methyl viologen was h...
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The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high-value antioxidant astaxanthin for over two decades, due to its superior ability to synthesize astaxanthin under adverse culture conditions. However, slow vegetative growth under favorable culture conditions and cell deterioration or death under stress conditions (e.g., high light, nitroge...
متن کاملRegulation of two carotenoid biosynthesis genes coding for phytoene synthase and carotenoid hydroxylase during stress-induced astaxanthin formation in the green alga Haematococcus pluvialis.
Astaxanthin is a high-value carotenoid used as a pigmentation source in fish aquaculture. In addition, a beneficial role of astaxanthin as a food supplement for humans is becoming evident. The unicellular green alga Haematococcus pluvialis seems to be a suitable source for natural astaxanthin. Astaxanthin accumulation in H. pluvialis occurs in response to environmental stress such as high light...
متن کاملFour Different Methods Comparison for Extraction of Astaxanthin from Green Alga Haematococcus pluvialis
Haematococcus pluvialis is one of the potent organisms for production of astaxanthin. Up to now, no efficient method has been achieved due to its thick cell wall hindering solvent extraction of astaxanthin. In this study, four different methods, hydrochloric acid pretreatment followed by acetone extraction (HCl-ACE), hexane/isopropanol (6:4, v/v) mixture solvents extraction (HEX-IPA), methanol ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2006
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01461-06